Why nuclease free water

Show More. Nuclease-Free Water 5 liters. Log in to see your account pricing. The Nuclease-Free Water is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease. Resources Safety Data Sheets 1. Safety Data Sheets EN. Kit Handbooks 2. PDF KB. PDF 33KB. Nuclease contamination in reagents used for nucleic acid isolation and analysis can contribute to experimental inconsistency and sometimes even experimental failure.

In trying to pinpoint the sources of contamination, it is easy to overlook the water used either to prepare reagents or to resuspend precipitated RNA. Even purified water can have a high pH and minerals that can interfere with reactions that require specific salt and pH conditions. We offer several brands and grades of nuclease-free water—diethylpyrocarbonate DEPC -treated water, nuclease-free water not DEPC-treated , and RT-PCR grade water—that all have been rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.

To meet the special challenges of working with RNA, our Invitrogen waters are certified nuclease-free. They are Type II megohm filtered via state-of-the-art reverse osmosis filtration and deionization , followed by autoclaving and sterile filtration. The purification equipment used to produce Invitrogen waters is subjected to routine maintenance and testing procedures according to rigid ISO specifications.

StemCell Technologies Inc. You can change your email preferences at any time. Autoclavable, UV-resistant, multi-channel pipettor designed for universal fit. Nuclease-Free Water. DNase- and RNase-free water for molecular biology applications. Size Choose an Option Size mL. Size 2 mL. Size 10 x 2 mL. From: 12 USD. Add to Cart.